主讲人：Dr. Weerawat Runguphan
Runguphan 博士2006年在哈佛大学获得Chemistry and Physics学士学位(Magna Cum Laude with Highest Honors)，2011年在MIT获得生物化学博士学位，年在美国Lawrence Berkeley National Lab从事合成生物学相关博士后研究工作，合作导师Jay Keasling教授。2014年起在泰国国家遗传工程与生物技术研究所工作，任课题组长。主要从事酿酒酵母代谢工程改造和生物燃料生产相关研究。
Interests in renewable fuels have exploded in recent years as the serious effects of global climate change become apparent. Microbial production of high-energy fuels by economically efficient bioprocesses has emerged as an attractive alternative to the traditional production of transportation fuels. Here, we highlight two examples of engineering yeast systems to produce biofuels. In the first example, we engineered the budding yeast Saccharomyces cerevisiae to produce fatty acid-derived biofuels and chemicals from simple sugars. Our engineered strains were able to produce free fatty acids at a titer of approximately 400mg/L, fatty alcohols at approximately 100 mg/L and fatty acid ethyl esters (biodiesel) at approximately 5 mg/L directly from simple sugars. We further optimized our original production strains by increasing the cytosolic pool of NADPH, a redox cofactor that is essential in fatty acid biosynthesis. In the second example, we engineered Pichia pastoris, an industrial workhorse in heterologous enzyme production, to produce the biofuels isobutanol and isopentanol from two renewable carbon sources from renewable carbon sources. Our strategy exploited the yeast’s amino acid biosynthetic pathway and diverted the amino acid intermediates to the 2-keto acid degradation pathway for higher alcohol production. Rewiring of the cells' primary metabolism using CRISPR/Cas9 further improved product yields. Efforts are underway to create a consolidated bioprocessing platform based on a single microorganism that directly converts sugarcane bagasse, one of the most abundant agricultural wastes in Thailand, into advanced biofuels. We envision that our CBP platform will enable efficient utilization of agricultural wastes and provide an economic route to production of advanced biofuels and chemicals.
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